β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer

Reporter gene assay β-galactosidase substrates - SK-Hep-1

Experiment
Reporter gene assay β-galactosidase substrates - SK-Hep-1
Product
β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer from Promega
Manufacturer
Promega

Protocol tips

Protocol tips
Add 150µl of Assay 2X Buffer to sample.

Incubate the reactions at 37°C for 30 minutes or until a faint yellow color has developed.
Downstream tips
Read the absorbance at 420nm.

Publication protocol

Luciferase activity assay was performed as previously described with slight modifications . Briefly, SK-Hep-1 cells were cultured in 24-well dishes, and then transiently co-transfected with 1 µg of pGL3-uPA luciferase reporter constructs and 1 µg of β-galactosidase reporter plasmid with the Lipofectamine 2000 transfection reagent, followed by various concentration of LicA for 24 h. Luciferase and β-galactosidase activities were determined with the luciferase and β-galactosidase enzyme assay system (Promega). The luminescence was measured using multilabel plate reader (Perkin Elmer). Luciferase activity was normalized to the β-galactosidase activity in cell lysates, and the mean of three independent experiments was calculated

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Reporter gene assay β-galactosidase substrates - SK-Hep-1 using β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer from Promega.

Paper title
Licochalcone A Suppresses Migration and Invasion of Human Hepatocellular Carcinoma Cells through Downregulation of MKK4/JNK via NF-κB Mediated Urokinase Plasminogen Activator Expression
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Manufacturer protocol

Download the product protocol from Promega for β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer below.

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