β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add 150µl of Assay 2X Buffer to sample. Incubate the reactions at 37°C for 30 minutes or until a faint yellow color has developed.	Read the absorbance at 420nm.

Protocol tips
Add 150µl of Assay 2X Buffer to sample. Incubate the reactions at 37°C for 30 minutes or until a faint yellow color has developed.
Downstream tips
Read the absorbance at 420nm.

Publication protocol

Luciferase activity assay was performed as previously described with slight modifications . Briefly, SK-Hep-1 cells were cultured in 24-well dishes, and then transiently co-transfected with 1 µg of pGL3-uPA luciferase reporter constructs and 1 µg of β-galactosidase reporter plasmid with the Lipofectamine 2000 transfection reagent, followed by various concentration of LicA for 24 h. Luciferase and β-galactosidase activities were determined with the luciferase and β-galactosidase enzyme assay system (Promega). The luminescence was measured using multilabel plate reader (Perkin Elmer). Luciferase activity was normalized to the β-galactosidase activity in cell lysates, and the mean of three independent experiments was calculated

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Reporter gene assay β-galactosidase substrates - SK-Hep-1 using β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer from Promega.

Manufacturer protocol

Download the product protocol from Promega for β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Reporter gene assay β-galactosidase substrates - SK-Hep-1 using β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer from Promega. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.