Senescence β-Galactosidase Staining Kit - Beyotime

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add 1 ml of the β-Galactosidase Staining Solution Incubate overnight at 37 ° C without Co2

Protocol tips
Add 1 ml of the β-Galactosidase Staining Solution Incubate overnight at 37 ° C without Co2

Publication protocol

Senescent cells were analyzed using a SA -gal staining kit (Beyotime Inc., Nantong, China) according to the manufacturer's instructions. The percentage of SA--gal positive cells was calculated by counting the cells in 5 random fields (at least 100 cells) using bright-field microscopy, according to the method reported by te Poele et al [].

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