Senescence Cells Histochemical Staining Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Fix cells in 2% paraformaldehyde and 0.02% glutaraldehyde in PBS Add 1 ml of the Staining Mixture per well and incubate for 16 h at 37 °C without CO2 until the cells are stained blue

Protocol tips
Fix cells in 2% paraformaldehyde and 0.02% glutaraldehyde in PBS Add 1 ml of the Staining Mixture per well and incubate for 16 h at 37 °C without CO2 until the cells are stained blue

Publication protocol

SA‐β‐gal‐positive cells were detected applying the Senescence Cells Histochemical Staining Kit obtained from Sigma‐Aldrich. In brief, cells were fixed with 2% paraformaldehyde and 0.02% glutaraldehyde in PBS and incubated for 16 hr in staining solution containing X‐Gal (5 mM C6N6FeK4/5 mMC6N6FeK3/2 mM MgCl2/1 mg/ml 5‐bromo‐4‐chloro‐3‐indolyl‐β‐d‐galacto‐pyranoside in PBS). SA‐β‐gal positive cells as percentage of total cell counts were calculated using an Olympus IX50 inverted microscope (Olympus, Center Valley, PA). For photometrical SA‐β‐gal detection, cells were fixed and incubated in staining solution supplemented with 0.2 mM fluorescein di‐β‐d‐galactopyranoside (FDG) 24 hr before fluorescence determination (excitation wave length: 485 nm, emission wave length: 535 nm).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Reporter gene assay β-galactosidase substrates - HepG2 using Senescence Cells Histochemical Staining Kit from Sigma-Aldrich.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Senescence Cells Histochemical Staining Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Reporter gene assay β-galactosidase substrates - HepG2 using Senescence Cells Histochemical Staining Kit from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.