Senescence Cells Histochemical Staining Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add 1 ml of the Staining Mixture per well and incubate at 37 °C without CO2 until the cells are stained blue.	Measure for absorbance at 420 nm

Protocol tips
Add 1 ml of the Staining Mixture per well and incubate at 37 °C without CO2 until the cells are stained blue.
Downstream tips
Measure for absorbance at 420 nm

Publication protocol

As an indicator of senescence in cells, SA- β-galactosidase staining was performed, using Senescence Cells Histochemical Staining Kit (Sigma-Aldrich, Germany). After staining, according to the manufacturer's protocol, supernatants were collected and measured for absorbance at 420 nm. The number of viable cells and dead cells was assessed using Cell stain Double Staining Kit (Sigma-Aldrich, Germany). The nuclei of dead cells were stained with propidium iodide, whereas the nuclei of viable cells were stained with Calcein-AM. Cells were then observed using epifluorescence microscopy. The number of dead cells was calculated as percentage of all viable cells. All procedures were performed in accordance to manufacturer's instructions.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Senescence Cells Histochemical Staining Kit below.

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