Mammalian beta-Galactosidase Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 5 × 10^4 cells	Add 100µL of β-Galactosidase Assay Reagent. Cover plate and incubate for 30 minutes at 37°C.	Measure absorbance at 405nm.

Upstream tips
Seed 5 × 10^4 cells
Protocol tips
Add 100µL of β-Galactosidase Assay Reagent. Cover plate and incubate for 30 minutes at 37°C.
Downstream tips
Measure absorbance at 405nm.

Publication protocol

For transfection, Panc-1, Aspc-1, and Bxpc-3 cells were seeded at 5 × 104 cells per well in 24-well plates. The β-galactosidase plasmid (50 ng) and pGL3-FBP1- Luc reporter (200 ng), along with a pcDNA3.1-Flag-NPM1 plasmid, were transiently transfected into the cells. Twenty-four hours post-transfection, cells were harvested and lysed. The luciferase and β-galactosidase activities were measured with a luciferase reporter gene assay kit (Invitrogen) and a β-galactosidase kit (Invitrogen), respectively. The transfection efficiency across plates was normalized to β-galactosidase activity, and all transfections were repeated three times in duplicate.

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Manufacturer protocol

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