Senescence β-Galactosidase Staining Kit - Cell Signaling

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Fix cells in 1x fixation solution containing 2% formaldehyde and 0.2% glutaraldehyde in PBS	Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2).

Upstream tips
Fix cells in 1x fixation solution containing 2% formaldehyde and 0.2% glutaraldehyde in PBS
Protocol tips
Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2).

Publication protocol

For senescence assay cells were seeded in 6-well plates. After washing with PBS, cells were incubated with 1x fixation solution containing 2% formaldehyde and 0.2% glutaraldehyde in PBS. The cells were washed again and stained for 4 hr with 1 mg/ml X-gal, 40 mM citric acid/sodium phosphate (pH 6.0), 150 mM NaCl, and 2 mM MgCl2. The stain was removed, cells were rinsed with PBS, and positive staining was viewed and scored.

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for Senescence β-Galactosidase Staining Kit - Cell Signaling below.

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