ZR RNA MiniPrepTM kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- The kit is designed for efficient isolation of total RNA from 1x10^2to 1x10^7 cells.

Upstream tips
- The kit is designed for efficient isolation of total RNA from 1x10^2to 1x10^7 cells.

Publication protocol

RNA was isolated with an miRNeasy Mini Kit (Qiagen,
Basel, Switzerland) for islet cells and with the ZR RNA MiniPrep kit (Zymo Research, Irvine, CA) for INS832/13
cells. The samples were treated with DNase (Promega, Madison, WI) before retrotranscription using RNAse H minus (Promega), a Maloney murine leukemia virus reverse transcriptase. To quantify lncRNAs and mRNAs, realtime PCR was performed with the use of iQ SYBR Green Supermix.

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Manufacturer protocol

Download the product protocol from Zymo Research for ZR RNA MiniPrepTM kit below.

Download manufacturer protocol

Videos

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