ZR RNA MiniPrepTM kit

RNA isolation / purification Cells - primary human islets of langerhans

Experiment
RNA isolation / purification Cells - primary human islets of langerhans
Product
ZR RNA MiniPrepTM kit from Zymo Research
Manufacturer
Zymo Research

Protocol tips

Upstream tips
- The kit is designed for efficient isolation of total RNA from 1x10^2to 1x10^7 cells.

Publication protocol

RNA was isolated with an miRNeasy Mini Kit (Qiagen,
Basel, Switzerland) for islet cells and with the ZR RNA MiniPrep kit (Zymo Research, Irvine, CA) for INS832/13
cells. The samples were treated with DNase (Promega, Madison, WI) before retrotranscription using RNAse H minus (Promega), a Maloney murine leukemia virus reverse transcriptase. To quantify lncRNAs and mRNAs, realtime PCR was performed with the use of iQ SYBR Green Supermix.

Full paper   Login or join for free to view the full paper.

Reviews

ZR RNA MiniPrepTM kit from Zymo Research has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human islets of langerhans using ZR RNA MiniPrepTM kit from Zymo Research.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Zymo Research for ZR RNA MiniPrepTM kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - primary human islets of langerhans using ZR RNA MiniPrepTM kit from Zymo Research. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms