β-Gal Reporter Gene Assay, chemiluminescent

Protocol tips

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	Wash cells and add β-Gal Reporter Gene Assay, incubate for 30 min at RT. Add substrate reagent and incubate for 15-60 min at RT

Protocol tips
Wash cells and add β-Gal Reporter Gene Assay, incubate for 30 min at RT. Add substrate reagent and incubate for 15-60 min at RT

Publication protocol

Cells were incubated in serum-free medium, with (F2-27 or control isotype mAb) or without antibody, at 37°C in 5% CO2 for 1 hour. After incubation, cells were cultured in serum-free medium containing Ax3CAZ3-FZ33 (104 VP/cell) at 37°C in 5% CO2 for 1 hour. After incubation, cells were washed and then cultured in RPMI-1640 with 10% FBS for 24 hours. LacZ activity was measured by a β-gal reporter gene assay (Roche Molecular Biochemicals, Indianapolis, IN).

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for β-Gal Reporter Gene Assay, chemiluminescent below.

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