Senescence β-Galactosidase Staining Kit - Cell Signaling

Protocol tips

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	Fix cells with Fixative Solution for 15 minutes at room temperature, Incubate overnight (cells) at 37 ° C without Co2 and 48 hours (frozen sections)

Protocol tips
Fix cells with Fixative Solution for 15 minutes at room temperature, Incubate overnight (cells) at 37 ° C without Co2 and 48 hours (frozen sections)

Publication protocol

β-galactosidase staining was performed using Senescence β-Galactosidase Staining Kit (Cat#9860, Cell Signaling Technology)13, following the manufacturers’ instructions. Briefly, HCC cells grown in 6-well plates or frozen sections from xenograft tissues were fixed with Fixative Solution for 15 minutes at room temperature, and then incubated with β-Galactosidase Staining Solution at 37 °C in a dry incubator without CO2 overnight (cell lines) or 48 hours (frozen sections). Slides were counterstained with Eosin. Cells or slides were examined under a Nikon microscope for the development of blue color.

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for Senescence β-Galactosidase Staining Kit - Cell Signaling below.

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