Senescence β-Galactosidase Staining Kit - Cell Signaling

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Pretreat cells with with or without H2S for 30 min and then stimulate with 50 μM H2O2 for 1 h.	Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)

Upstream tips
Pretreat cells with with or without H2S for 30 min and then stimulate with 50 μM H2O2 for 1 h.
Protocol tips
Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)

Publication protocol

Cells were pretreated with or without H2S for 30 min followed by stimulation with 50 μM H2O2 for 1 h, then transferred to normal 10% serum DMEM and cultured continually for 24 h. Cellular senescence was detected using a Senescence-β-galactosidase staining kit from Cell Signaling Technology according to the manufacturer's protocol. Images were acquired with a Nikon light microscope. SA-β-gal-positive cells were stained as blue color

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for Senescence β-Galactosidase Staining Kit - Cell Signaling below.

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