Senescence β-Galactosidase Staining Kit - Cell Signaling

Reporter gene assay β-galactosidase substrates - SH-SY5Y

Experiment
Reporter gene assay β-galactosidase substrates - SH-SY5Y
Product
Senescence β-Galactosidase Staining Kit - Cell Signaling from Cell Signaling Technology
Manufacturer
Cell Signaling Technology

Protocol tips

Upstream tips
Pretreat cells with with or without H2S for 30 min and then stimulate with 50 μM H2O2 for 1 h.

Protocol tips
Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)

Publication protocol

Cells were pretreated with or without H2S for 30 min followed by stimulation with 50 μM H2O2 for 1 h, then transferred to normal 10% serum DMEM and cultured continually for 24 h. Cellular senescence was detected using a Senescence-β-galactosidase staining kit from Cell Signaling Technology according to the manufacturer's protocol. Images were acquired with a Nikon light microscope. SA-β-gal-positive cells were stained as blue color

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Reporter gene assay β-galactosidase substrates - SH-SY5Y using Senescence β-Galactosidase Staining Kit - Cell Signaling from Cell Signaling Technology.

Paper title
Sulfhydration of p66Shc at Cysteine59 Mediates the Antioxidant Effect of Hydrogen Sulfide
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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for Senescence β-Galactosidase Staining Kit - Cell Signaling below.

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