Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add diluted Galacton-Plus substrate 1:100 with Reaction Buffer Diluent. Add 70µL of Reaction Buffer, mix, and incubate for 30-60 min

Protocol tips
Add diluted Galacton-Plus substrate 1:100 with Reaction Buffer Diluent. Add 70µL of Reaction Buffer, mix, and incubate for 30-60 min

Publication protocol

RAW 264.7 cells stably transfected with Lgr4 shRNA were transfected with NF-κB-luciferase reporter (pNF-κB-luc, Stratagene) with Lipofectamine 2000 (Invitrogen) as described by the manufacturer. Then, 48 h after transfection, cells were stimulated with different TLR agonists, and luciferase activity was measured with the Dual-Luciferase reporter assay system (Promega). Extracts were also normalized by β-galactosidase activity using the Galacto-Light Plus β-galactosidase reporter gene assay system (Promega).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System below.

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