Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System

Reporter gene assay β-galactosidase substrates - C2C12

Experiment
Reporter gene assay β-galactosidase substrates - C2C12
Product
Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
Seed ~ 2 × 105 cells/well
Protocol tips
Add diluted Galacton-Plus substrate 1:100 with Reaction Buffer Diluent.

Add 70µL of Reaction Buffer, mix, and incubate for 30-60 min

Publication protocol

C2C12 cells were grown in a 24-well plate (~ 2 × 105 cells/well) in DMEM supplemented with 10% FBS. After reaching approximately 70% confluence, cells were transfected using Superfect transfection reagent (Qiagen, Hilden, Germany) with 500 ng/well of each specific luciferase construction and 25 ng/well of SV40-β-Gal as an internal control for transfection efficiency. Six hours after transfection, the cells were fed with fresh medium (DMEM with 10% FBS) and incubated overnight. Transfected cells were then treated for 48 h, as indicated in specific experiments. Cell extracts were then prepared and luciferase assays were done using the Luciferase Assay System (Promega, Madison, WI, USA). Luciferase activities were normalized with respect to parallel beta-galactosidase activities, to correct for differences in transfection efficiency. Beta-galactosidase assays were performed using the Galacto-Light Plus System from Tropix (Bedford, MA, USA).

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Reporter gene assay β-galactosidase substrates - C2C12 using Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System from Thermo Fisher Scientific.

Paper title
Interleukin 17 inhibits myogenic and promotes osteogenic differentiation of C2C12 myoblasts by activating ERK1,2.
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System below.

Download PDF Download manufacturer protocol

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