Senescence β-Galactosidase Staining Kit - Cell Signaling

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Fix cells after 72 hrs of miR-7 transfection.	Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)

Upstream tips
Fix cells after 72 hrs of miR-7 transfection.
Protocol tips
Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)

Publication protocol

Cells were fixed 72 hrs after miR-7 transfection or 96 hrs after BrdU addition (#B5002-100MG, Sigma-Aldrich). β -galactosidase activity was assayed as recommended in the senescence β-galactosidase staining kit (#9860S, Cell Signalling).

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for Senescence β-Galactosidase Staining Kit - Cell Signaling below.

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