Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Dilute Galacton-Plus substrate 1:100 with reaction buffer diluent to make a reaction buffer. Add 70 ul of this buffer and incubate for 30-60 min

Protocol tips
Dilute Galacton-Plus substrate 1:100 with reaction buffer diluent to make a reaction buffer. Add 70 ul of this buffer and incubate for 30-60 min

Publication protocol

Transfected CHO-K1 cells were infected with type 5 AdV-β-Gal at a multiplicity of infection of 100 for 1 hr at 37°C. β-galactosidase expression was determined with Galacto-Light Plus System (Applied Biosystems, Carlsbad, CA), as previously described (Excoffon, Gansemer et al. 2010, Kolawole, Sharma et al. 2012).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System below.

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