High-capacity cDNA reverse transcription kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
This Kit uses the random primer scheme for initiating cDNA synthesis.	Add 10 μL of 2X RT master mix and 10 μL of RNA sample and mix. Treat tubes at 25C for 10 minutes, 37C for 2h and 85C for 5 minutes.

Upstream tips
This Kit uses the random primer scheme for initiating cDNA synthesis.
Protocol tips
Add 10 μL of 2X RT master mix and 10 μL of RNA sample and mix. Treat tubes at 25C for 10 minutes, 37C for 2h and 85C for 5 minutes.

Publication protocol

cDNA was prepared using High Capacity cDNA Reverse Transcription Kit (4368814, Applied Biosystems) following the manufacturer’s protocol. RT-PCR was performed using FAST SYBR kit (4385616, Applied Biosystems) with three technical replicates and analyzed on an Applied Biosystems 7500 FAST and Real-Time PCR system. The fold change of gene expression was calculated using the 2^(-ΔΔCt) method. Primers used in qPCR are listed in Table S1C.

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Manufacturer protocol

Download the product protocol from Applied Biosystems for High-capacity cDNA reverse transcription kit below.

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