Senescence Cells Histochemical Staining Kit

Protocol tips

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	Fix cells for 7 min in 1×fixative solution. Add 1 ml of the Staining Mixture per well and incubate for 48 h at 37 °C without CO2 until the cells are stained blue

Protocol tips
Fix cells for 7 min in 1×fixative solution. Add 1 ml of the Staining Mixture per well and incubate for 48 h at 37 °C without CO2 until the cells are stained blue

Publication protocol

Cells were stained using the Senescence Cells Histochemical Staining Kit (Sigma Aldrich, St Louis, MO, USA) following the manufacturer's instructions. In brief, cells were washed once in 1×PBS, fixed for 7 min in 1×fixative solution, and washed twice in 1×PBS, follow by stained with fresh SA-β-gal staining solution at 37°C for 48h. The percentage of positively stained cell (blue cells) versus total cells was counted by randomly choosing six microscopic fields. The images were captured at 100× magnification using a Nikon Eclipse TS100 microscope (Nikon Corporation, Tokyo, Japan).

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Senescence Cells Histochemical Staining Kit below.

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