Senescence β-Galactosidase Staining Kit - Cell Signaling

Reporter gene assay β-galactosidase substrates - mouse pancreatic stellate cells

Experiment
Reporter gene assay β-galactosidase substrates - mouse pancreatic stellate cells
Product
Senescence β-Galactosidase Staining Kit - Cell Signaling from Cell Signaling Technology
Manufacturer
Cell Signaling Technology

Protocol tips

Protocol tips
Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)

Publication protocol

PSCs were cultured on coverslips for 2 days as indicated. Afterwards, SA β-Gal-positive cells were stained
using a specific kit according to the instructions of the
manufacturer (New England BioLabs, Frankfurt, Germany). The nuclei were counterstained with nuclear fast red (Carl Roth, Karlsruhe, Germany). Subsequently, specimens were analyzed by light microscopy, and SA β-Galpositive and -negative cells were counted. All samples were evaluated in a blinded manner.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Reporter gene assay β-galactosidase substrates - mouse pancreatic stellate cells using Senescence β-Galactosidase Staining Kit - Cell Signaling from Cell Signaling Technology.

Paper title
Uncoupling protein 2 deficiency reduces proliferative capacity of murine pancreatic stellate cells.
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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for Senescence β-Galactosidase Staining Kit - Cell Signaling below.

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