Senescence β-Galactosidase Staining Kit - Cell Signaling

Protocol tips

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	Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)

Protocol tips
Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)

Publication protocol

PSCs were cultured on coverslips for 2 days as indicated. Afterwards, SA β-Gal-positive cells were stained
using a specific kit according to the instructions of the
manufacturer (New England BioLabs, Frankfurt, Germany). The nuclei were counterstained with nuclear fast red (Carl Roth, Karlsruhe, Germany). Subsequently, specimens were analyzed by light microscopy, and SA β-Galpositive and -negative cells were counted. All samples were evaluated in a blinded manner.

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for Senescence β-Galactosidase Staining Kit - Cell Signaling below.

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