Beta-Glo® Assay System

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Homogenize with reporter lysis buffer and centrifuged for 10 min at 12,000 g. Add β-Glo assay solution and incubate for 1 h at room temperature.

Protocol tips
Homogenize with reporter lysis buffer and centrifuged for 10 min at 12,000 g. Add β-Glo assay solution and incubate for 1 h at room temperature.

Publication protocol

The overall β-galactosidase activity was used to quantify the content of the LacZ-labeled MSCs in lungs, spleens, and livers of the animals in the 24 h biodistribution studies. Briefly, the organs were homogenized with 1 mL of reporter lysis buffer (Promega®, Madison, WI)/0.5 g of tissue and centrifuged for 10 min at 12,000 g. The supernatant was then used to measure β-galactosidase activity with the β-Glo assay solution (Promega) after 1 h of incubation at room temperature.20 The relative light units (RLUs) were measured with a luminometer (PerkinElmer, Boston, MA). The data (β-galactosidase activity/gram of tissue) is represented as absolute RLU values within each organ and culture condition and as a ratio of the RLU in the spleens and livers relative to the lungs. The β-galactosidase enzyme activity in three uninjected rats was also measured in each organ and the average of these results was used as a baseline to subtract endogenous β-galactosidase activity from rats injected with LacZ+ MSCs.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Reporter gene assay β-galactosidase substrates - rat mesenchymal stem cells (MSCs) using Beta-Glo® Assay System from Promega.

Manufacturer protocol

Download the product protocol from Promega for Beta-Glo® Assay System below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Reporter gene assay β-galactosidase substrates - rat mesenchymal stem cells (MSCs) using Beta-Glo® Assay System from Promega. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.