Senescence β-Galactosidase Staining Kit - Beyotime

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 1 × 10^4 cells for 8-10 hours.	Incubate overnight at 37 ° C without Co2

Upstream tips
Seed 1 × 10^4 cells for 8-10 hours.
Protocol tips
Incubate overnight at 37 ° C without Co2

Publication protocol

NP cells seeded in the DBM scaffold were harvested as described above. After NP cells (1 × 104 per group) were subjected to adherent culture for 8–10 hours, SA-β-Gal staining was performed according to the manufacturer’s instructions (Senescence β-Galactosidase Staining Kit, Beyotime). The SA-β-Gal stained NP cells were observed under a light microscope (Olympus BX51; Olympus, Tokyo, Japan), and then NP cell senescence, expressed as the percentage of senescent NP cells to total NP cells, was analysed with Image-Pro Plus software (Version 5.1, Media Cybernetics, Inc., Rockville, MD, USA).

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Manufacturer protocol

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