Senescence β-Galactosidase Staining Kit - Cell Signaling

Protocol tips

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	Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C for 12 hours in a dry incubator (no CO2)

Protocol tips
Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C for 12 hours in a dry incubator (no CO2)

Publication protocol

The SA-β-gal staining of NP cells was investigated using SA-β-gal staining kit (Cell Signaling Technology). Fixative NP cells seeded in 12-well culture plates were incubated with the staining solution at 37°C for 12 h. Nine random fields per well were imaged using a phase-contrast microscope (200x magnification, Olympus). The mean percentage of SA-β-gal-positive cells was calculated.

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