JetPrime

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Always add your plasmid to the buffer before adding jetPrime

Upstream tips
Always add your plasmid to the buffer before adding jetPrime

Publication protocol

Luciferase assays
Primary chondrocytes were seeded at a density of 3 × 104 cells/well in 48 well plates 1 day prior to transfection. Cells were transfected with the pGL4.23-Mef2-luc (a gift from Eric Olson) or the pGL4.10-Ose2-luc reporter plasmid (a gift from G. Karsenty) in combination with pRL-TK (Promega) as a control and pCDNA3.1-DaCaMKII or pCDNA3.1-rKIIN expression vectors. Total amounts of transfected plasmids (equivalent to 52 fM) in each group were adjusted by adding empty vector (pCDNA3.1+). Transfection was performed using jetPRIME™ (Polyplus transfection) according to manufacturer's instructions. Luciferase activities were measured 48 h after transfection following the protocol by (Hampf and Gossen, 2006). Luciferase measurements were normalized to the corresponding renilla activities for transfection efficiency. Experiments were performed in triplicates and repeated at least three times.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA transfection Mammalian cells - Primary cells Human chondrocytes using JetPrime from Polyplus transfections.

Manufacturer protocol

Download the product protocol from Polyplus transfections for JetPrime below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA transfection Mammalian cells - Primary cells Human chondrocytes using JetPrime from Polyplus transfections. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.