FuGENE® 6 Transfection Reagent

DNA transfection Mammalian cells - Primary cells Human chondrocytes

Experiment
DNA transfection Mammalian cells - Primary cells Human chondrocytes
Product
FuGENE® 6 Transfection Reagent from Promega
Manufacturer
Promega

Protocol tips

Upstream tips
Always bring FuGENE to room temperature and mix well prior to use.

Publication protocol

Chondrocyte transfection
The chondrocyte monolayers at 80% confluence were transfected with a mixture of the plasmid pCMV-SPORT6 EGFP and the FuGENE 6 transfection reagent (Roche Applied Bioscience, USA) using 1:3, 2:3, and 1:6 ratios of plasmid µg to FuGENE µL and incubated at 37°C in a 5% CO atmosphere for 48 h. The number of fluorescent chondrocytes was determined using a Nikon 50i microscope with an epifluorescence illuminator (Nikon Instruments Inc., USA) at a magnification of 20×. The transfection efficiency was reported as the percentage of fluorescent cells calculated with respect to the total number of cells observed in eight randomly selected microscopic fields. This experiment was performed in triplicate.

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Papers

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Paper title
Cotransfected human chondrocytes: over-expression of and enhances the synthesis of cartilage matrix components collagen-II and glycosaminoglycans
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Manufacturer protocol

Download the product protocol from Promega for FuGENE® 6 Transfection Reagent below.

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