|Always bring FuGENE to room temperature and mix well prior to use.
The chondrocyte monolayers at 80% confluence were transfected with a mixture of the plasmid pCMV-SPORT6 EGFP and the FuGENE 6 transfection reagent (Roche Applied Bioscience, USA) using 1:3, 2:3, and 1:6 ratios of plasmid µg to FuGENE µL and incubated at 37°C in a 5% CO atmosphere for 48 h. The number of fluorescent chondrocytes was determined using a Nikon 50i microscope with an epifluorescence illuminator (Nikon Instruments Inc., USA) at a magnification of 20×. The transfection efficiency was reported as the percentage of fluorescent cells calculated with respect to the total number of cells observed in eight randomly selected microscopic fields. This experiment was performed in triplicate. Full paper
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