Lipofectamine® 2000 Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Do not add antibiotics to the Transfection Medium

Protocol tips
Do not add antibiotics to the Transfection Medium

Publication protocol

Transfection of siRNA or miRNA knockdown probes.
The siRNAs used to silence human E2F3 were ON-TARGETplus SMARTpool siRNA (siE2F3) obtained from Dharmacon (Lafayette, CO). ON-TARGETplus SMARTpool Nontargeting siRNA were used as control (siCon). The siRNAs directly against human HIF-1α and HIF-2α (siHIF1α and siHIF2α) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). To knock down endogenous miR-210, anti-210 (miRCURY LNA microRNA Power inhibitor) and negative miRNA power inhibitor control (anti-Con) were used (obtained from Exiqon). In brief, cells at 80% confluence after overnight culture on 60-mm Petri dishes were transfected with 80 nM of siRNAs or miRNA inhibitors by Lipofectamine 2000 (Invitrogen). After 6 h of transfection, fresh medium was replaced, and the cells were cultured for 24 h and then exposed to hypoxia for 48 h.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA transfection Mammalian cells - Primary cells Human pulmonary artery smooth muscle cells (HPASMC) using Lipofectamine® 2000 Transfection Reagent from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Lipofectamine® 2000 Transfection Reagent below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA transfection Mammalian cells - Primary cells Human pulmonary artery smooth muscle cells (HPASMC) using Lipofectamine® 2000 Transfection Reagent from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.