Xfect™ Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Always add your plasmid to the buffer before adding Xfect Polymer Always keep the ratio of Polymer:DNA the same. Use 0.3 μl of Xfect Polymer per 1 μg of plasmid DNA.

Protocol tips
Always add your plasmid to the buffer before adding Xfect Polymer Always keep the ratio of Polymer:DNA the same. Use 0.3 μl of Xfect Polymer per 1 μg of plasmid DNA.

Publication protocol

Transfection of Plasmid DNA and Small Interfering RNA in hPASMCs
hPASMCs were transfected with SphK1 wild-type (WT) and G82D dominant-negative plasmids, or with human S1PR2 in pCMV6-XL4 vector (Cat.#SC117485; OriGene Technologies, Rockville, MD) using Xfect Transfection reagent (Cat.#631318; Clontech, Mountain View, CA). Scrambled small interfering RNA (siRNA) (D-001810-02) or S1PR2 siRNA (J-005208-06) were purchased from Dharmacon (Thermo Fisher Scientific, Lafayette, CO) and transfected into primary hPASMCs using DharmaFECT 1 transfection reagent (Thermo Fisher Scientific) according to the manufacturer’s protocol.

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Papers

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Manufacturer protocol

Download the product protocol from Takara Bio Inc for Xfect™ Transfection Reagent below.

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Videos

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