jetPEI®-HUVEC

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	jetPEI-HUVEC/DNA mixture was added drop-wise onto the M199M+Glutamax™ I medium (GIBCO) containing 2% FBS, mixed gently, and incubated in a humidified 37°C incubator for 4 h. The growth medium was then replaced and the cells were incubated further for 24 h.	To verify the transfection efficiency, HUVEC was transfected with Thy-1-pCMS-EGFP (enhanced green fluorescent protein)-C1, and then monitored using an inverted fluorescent microscope.

Protocol tips
jetPEI-HUVEC/DNA mixture was added drop-wise onto the M199M+Glutamax™ I medium (GIBCO) containing 2% FBS, mixed gently, and incubated in a humidified 37°C incubator for 4 h. The growth medium was then replaced and the cells were incubated further for 24 h.
Downstream tips
To verify the transfection efficiency, HUVEC was transfected with Thy-1-pCMS-EGFP (enhanced green fluorescent protein)-C1, and then monitored using an inverted fluorescent microscope.

Publication protocol

Cell Transfection
For transient transfection of the Thy-1 or constitutively active RhoA (RhoA V14) constructs into HUVEC, jetPEI-HUVEC transfection reagent (Polyplus Transfection, Bioparc, France) was used according to the manufacturer’s protocol. Briefly, a jetPEI-HUVEC/DNA mixture was added drop-wise onto the M199M+Glutamax™ I medium (GIBCO) containing 2% FBS, mixed gently, and incubated in a humidified 37°C incubator for 4 h. The growth medium was then replaced and the cells were incubated further for 24 h. To verify the transfection efficiency, HUVEC was transfected with Thy-1-pCMS-EGFP (enhanced green fluorescent protein)-C1, and then monitored using an inverted fluorescent microscope.

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Manufacturer protocol

Download the product protocol from Polyplus transfections for jetPEI®-HUVEC below.

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