Lipofectamine® LTX Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Do not add antibiotics to the Transfection Medium

Protocol tips
Do not add antibiotics to the Transfection Medium

Publication protocol

Inhibition of CaSR expression by specific siRNA
Transfection of CaSR siRNA (Santa Cruz Biotechnology) into HAoSMC was performed using lipofectamine (Invitrogen) according to manufacturer’s protocol. Control siRNA-A (Santa Cruz) and lipofectamine alone were used as negative controls. To maintain inhibition of the CaSR expression in longer-term cultures, transfection was repeated every 3 days. CaSR knockdown was monitored by Western blot. Following transfection the cells were cultured for 7 days and treated with 2 and 5mmol/L Ca, 50mol/L Gd or in combination.



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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Lipofectamine® LTX Reagent below.

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