SuperFect Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
SuperFect–DNA complex formation should be performed in the absence of serum, antibiotics, and proteins.

Upstream tips
SuperFect–DNA complex formation should be performed in the absence of serum, antibiotics, and proteins.

Publication protocol

2.8. Transient transfection and luciferase reporter assay
Sixty percent confluent cells were transfected with pAP-1-Luc or pNF-κB-Luc plasmids (PathDetect luciferase cis-reporter system containing 7 × AP1 and 5 × NF-κB enhancer elements, respectively, Stratagene) and pSV-β-Galactosidase control vector for transfection efficiency (Promega) using Superfect reagent (Qiagen). An empty luciferase plasmid, pLuc-MCS, was used as a control. After transfection, the cells were pretreated in serum-free maintenance medium for 16 h, and then treated with LDL for an additional 6 h. The cells were then harvested and the luciferase activities were measured using a Biolumat 9505 luminometer (Berthold) according to manufacturer's instructions (Promega). The luciferase activity was normalized to β-galactosidase activities. All experiments were conducted in triplicate.



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Manufacturer protocol

Download the product protocol from Qiagen for SuperFect Transfection Reagent below.

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