jetPEI® DNA transfection, HTS application

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Do not add antibiotics to the Transfection Medium

Protocol tips
Do not add antibiotics to the Transfection Medium

Publication protocol

ERK overexpression
Cells were transfected with human CMV6-XL4 MAPK (Origene, Rockville, MD, USA) or with the plasmid alone by the PEI method (JetPEI, Polyplus transfection, []). Two days after transfection, astrocytes were trypsinized and seeded in medium containing 200 μg/ml ampicillin. MAPK overexpression was verified by western blot, as described below.

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Manufacturer protocol

Download the product protocol from Polyplus transfections for jetPEI® DNA transfection, HTS application below.

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