Lipofectamine® 2000 Transfection Reagent

Protocol tips

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	Do not add antibiotics to the Transfection Medium

Protocol tips
Do not add antibiotics to the Transfection Medium

Publication protocol

ERα overexpression
For transfection experiments, the cells were plated in 6-well plates and maintained in phenol-red free DMEM containing 10% charcoal stripped FCS, and were transiently transfected with 2.5 μg of pSG5HEO (the wild-type human ERα expression vector) using LipofectamineTM 2000 transfection reagent (Thermo Fisher Scientific, Waltham, USA) in the presence of 10 nM 17-β-estradiol (Sigma-Aldrich). 48 h post transfection, cells were harvested and lysed for Western blot analysis.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Lipofectamine® 2000 Transfection Reagent below.

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