FuGENE® 6 Transfection Reagent

DNA transfection Mammalian cells - Primary cells Rat pulmonary artery smooth muscle cell (pPASMC)

Experiment
DNA transfection Mammalian cells - Primary cells Rat pulmonary artery smooth muscle cell (pPASMC)
Product
FuGENE® 6 Transfection Reagent from Promega
Manufacturer
Promega

Protocol tips

Upstream tips
Always bring FuGENE HD to room temperature and mix well prior to use.

Publication protocol

Luciferase Reporter Assay
RPASMCs were transfected with 500 ng plasmid containing nerve growth factor I-B response element–Luc and 50 ng of Renilla luciferase reporter plasmid pRL-RSV40 (Promega, Madison, WI) using FuGene 6 transfection reagent. At 24 hours after the transfection, cell lysates (20 μl) were assayed for luciferase activity with a dual-luciferase reporter assay system (Promega).



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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA transfection Mammalian cells - Primary cells Rat pulmonary artery smooth muscle cell (pPASMC) using FuGENE® 6 Transfection Reagent from Promega.

Paper title
Nur77 Suppresses Pulmonary Artery Smooth Muscle Cell Proliferation through Inhibition of the STAT3/Pim-1/NFAT Pathway
Full paper
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Manufacturer protocol

Download the product protocol from Promega for FuGENE® 6 Transfection Reagent below.

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