FuGENE® 6 Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Always bring FuGENE HD to room temperature and mix well prior to use.

Upstream tips
Always bring FuGENE HD to room temperature and mix well prior to use.

Publication protocol

Luciferase Reporter Assay
RPASMCs were transfected with 500 ng plasmid containing nerve growth factor I-B response element–Luc and 50 ng of Renilla luciferase reporter plasmid pRL-RSV40 (Promega, Madison, WI) using FuGene 6 transfection reagent. At 24 hours after the transfection, cell lysates (20 μl) were assayed for luciferase activity with a dual-luciferase reporter assay system (Promega).



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Papers

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Manufacturer protocol

Download the product protocol from Promega for FuGENE® 6 Transfection Reagent below.

Download manufacturer protocol

Videos

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