FuGENE® 6 Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Always bring FuGENE 6 to room temperature and mix well prior to use.

Upstream tips
Always bring FuGENE 6 to room temperature and mix well prior to use.

Publication protocol

Cloning and transfection
pCEP4/HIF-1α construct deriving from human HIF-1α cDNA sequence was purchased from ATCC (Manassas, VA, USA). The cardiomyocytes were incubated in serum-containing medium at 37°C for 24 hrs, then subjected to transfection. Before transfection, medium was changed to serum-free MEM for 2–4 hrs. Cardiomyocytes were transiently transfected with 1.6 μg of vectors carrying HIF-1α or empty vectors using FuGene 6 (Roche Diagnostics, Alameda, CA, USA) according to the manufacturer’s instructions. The cardiomyocytes, 44 hrs after transfection, were subjected to MH (10% O) for 0, 1, 3, 6 or 12 hrs. pcDNA3.1 empty vector transfected cells in every single experiment, acts as negative control (NC).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA transfection Mammalian cells - Primary cells Rat articular chondrocytes using FuGENE® 6 Transfection Reagent from Promega.

Manufacturer protocol

Download the product protocol from Promega for FuGENE® 6 Transfection Reagent below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA transfection Mammalian cells - Primary cells Rat articular chondrocytes using FuGENE® 6 Transfection Reagent from Promega. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.