Lipofectamine® 2000 Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Do not add antibiotics to the Transfection Medium

Protocol tips
Do not add antibiotics to the Transfection Medium

Publication protocol

Cell culture and plasmids transfection
Rat microglia were purchased from Sciencell Research Laboratory, California, USA. They were grown at 37°C in 5% CO in DMEM (high glucose) supplemented with 10% FBS, penicillin at 100 units/ml and streptomycin at 100 µg/ml. Media were changed every 2 to 3 days after plating. The adherent cells were digested by D-Hank's containing 0.25% trypsogen, plated in cell plates at suitable densities, and incubated at 37°C in 5% CO until treatment. Vector and tau40 plasmids were transfected into microglia using Lipofectamine 2000 according to the protocol of the manufacturer (Invitrogen). At 24 h after transfection, cells were collected and used for further studies. By counting of the plated cells, the transfection efficiencies of the plasmids (vector and tau40) in this study were about 40%.



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Manufacturer protocol

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