FuGENE® 6 Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Always bring FuGENE 6 to room temperature and mix well prior to use.

Upstream tips
Always bring FuGENE 6 to room temperature and mix well prior to use.

Publication protocol

Cloning and transfection
pCEP4/HIF-1α construct deriving from human HIF-1α cDNA sequence was purchased from ATCC (Manassas, VA, USA). The cardiomyocytes were incubated in serum-containing medium at 37°C for 24 hrs, then subjected to transfection. Before transfection, medium was changed to serum-free MEM for 2–4 hrs. Cardiomyocytes were transiently transfected with 1.6 μg of vectors carrying HIF-1α or empty vectors using FuGene 6 (Roche Diagnostics, Alameda, CA, USA) according to the manufacturer’s instructions. The cardiomyocytes, 44 hrs after transfection, were subjected to MH (10% O) for 0, 1, 3, 6 or 12 hrs. pcDNA3.1 empty vector transfected cells in every single experiment, acts as negative control (NC).

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Papers

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Manufacturer protocol

Download the product protocol from Promega for FuGENE® 6 Transfection Reagent below.

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