FuGENE® HD Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Always bring FuGENE HD to room temperature and mix well prior to use.

Upstream tips
Always bring FuGENE HD to room temperature and mix well prior to use.

Publication protocol

Transfection.
Mesangial cells were transfected with plasmid vectors or siRNAs targeted against Erk5 using FuGENE HD essentially as described previously (14, 15). For siRNA transfection, scrambled RNA was used as control.

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Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA transfection Mammalian cells - Primary cells Rat mesangial cells using FuGENE® HD Transfection Reagent from Promega.

Manufacturer protocol

Download the product protocol from Promega for FuGENE® HD Transfection Reagent below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA transfection Mammalian cells - Primary cells Rat mesangial cells using FuGENE® HD Transfection Reagent from Promega. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.