FuGENE® HD Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Always bring FuGENE HD to room temperature and mix well prior to use.

Upstream tips
Always bring FuGENE HD to room temperature and mix well prior to use.

Publication protocol

The transfection was mediated by FuGENE® HD transfection reagent (Promega E2311). All the procedures of transfection were performed according to the manufacturer’s instructions. Briefly, add 2 μg or 4 μg of plasmid DNA to a sterile tube, and vortex, for a 3:1 FuGENE® HD Transfection Reagent: DNA ratio, add 6 μl or 8 μl of FuGENE® HD Transfection Reagent directly to medium, and mix immediately. Incubate for 0-15 minutes. Add the mixture to H9c2 cardiomyoblasts in six-well plate, evaluate the transfection efficiency 36 hours later using Western Blot. Finally, 4 μg plasmid DNA was used in further experiments.

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Manufacturer protocol

Download the product protocol from Promega for FuGENE® HD Transfection Reagent below.

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