X-tremeGENE™ HP DNA Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Do not aliquot X-tremeGENE HP DNA Transfection Reagent; store in the original glass vials.

Upstream tips
Do not aliquot X-tremeGENE HP DNA Transfection Reagent; store in the original glass vials.

Publication protocol

Transfection
In order to transfect the vector, plasmid DNA was diluted with serum-free medium in a sterile tube and gently pipetted to mix. X-tremeGENE HP DNA Transfection Reagent (cat# 06366236001; Roche, Germany) was then added to the diluted DNA and incubated at 15°C–25°C for 15 min. The cells were transfected with either miR-590-pLenti-III-eGFP or the control vector. This transfection complex was added to the cells in a drop-wise manner. The wells were gently shaken and swirled to ensure even distribution over the entire plate. After incubation for 72 h, the expression levels of the candidate miRNA and candidate genes were measured by real-time PCR.

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Discussion

Papers

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for X-tremeGENE™ HP DNA Transfection Reagent below.

Download manufacturer protocol

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