X-tremeGENE™ HP DNA Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Do not aliquot X-tremeGENE HP DNA Transfection Reagent; store in the original glass vials.

Upstream tips
Do not aliquot X-tremeGENE HP DNA Transfection Reagent; store in the original glass vials.

Publication protocol

Transient RNA interference and transfections
GRIM-19 was knocked down using small siRNAs, with a non-targeting siRNA used in parallel as a negative control (GenePharma Co., Shanghai, China). Primary cultures were transfected with a GRIM-19 siRNA or an irrelevant siRNA (as a control) using the X-tremeGENE HP DNA transfection reagent (Roche Diagnostics GmbH, Mannheim, Germany). After 2 days, the protein expression levels of GRIM-19, p-Stat3, Stat3 and β-actin in HeLa cells were analyzed.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for X-tremeGENE™ HP DNA Transfection Reagent below.

Download manufacturer protocol

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