Lipofectamine® LTX Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Do not add antibiotics to the Transfection Medium

Protocol tips
Do not add antibiotics to the Transfection Medium

Publication protocol

4. Transfection
Cells were plated 24 hours prior to transfection at a density of 2×10 cells per 35 mm culture dish. Cells were transiently transfected using Lipofectamine LTX (Invitrogen, Carlsbad, CA, USA) and 2.5 µg of each HDAC isoform expression vector or mock vector as a control. Twenty-four hours after transfection, the cells were treated with cisplatin for the indicated length of time.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Lipofectamine® LTX Reagent below.

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