In Situ Cell Death Detection Kit, TMR red

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Fix cell samples with Fixation solution for 1 h. Incubate in Permeabilisation solution (0.1% Triton X-100 in PBS) for 2 min on ice. Label and incubate for 60 min at +37°C in a humidified atmosphere in the dark	If low labelling is found, reduce fixation time or try 2% buffered paraformaldehyde

Protocol tips
Fix cell samples with Fixation solution for 1 h. Incubate in Permeabilisation solution (0.1% Triton X-100 in PBS) for 2 min on ice. Label and incubate for 60 min at +37°C in a humidified atmosphere in the dark
Downstream tips
If low labelling is found, reduce fixation time or try 2% buffered paraformaldehyde

Publication protocol

The In situ Cell Death Detection Kit-TMR (Roche) was used to detect cell apoptosis following the manufacture’s instruction. The pictures were taken under a fluorescent microscope and the percentage of positive cells was calculated using Image J software (NIH). Four fields were randomly chosen from each well of 12-well plates for the analysis.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for In Situ Cell Death Detection Kit, TMR red below.

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