Cell Cycle and Apoptosis Analysis Kit

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	- Cells were treated with 20 µg/mL RNase, stained with 20 µg/mL propidium iodide

Protocol tips
- Cells were treated with 20 µg/mL RNase, stained with 20 µg/mL propidium iodide

Publication protocol

Cell cycle analysis was performed by using Cell Cycle and Apoptosis Analysis Kits (Beyotime). HT-29 cells were seeded at a density of approximately 5×105 cells/well into 12-well plates, cultured for 48 h after transfection as mentioned above, harvested, washed with PBS, and then fixed with 70% ethanol overnight at 4°C. Cells were treated with 20 µg/mL RNase, stained with 20 µg/mL propidium iodide for 30 min at 37°C in the dark, and then analyzed by flow cytometry (FACScan; BD Biosciences, San Diego, CA, USA).


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