Muse® Cell Cycle Assay Kit

Protocol tips

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	- After seeding cells are treated with 0.1% DMSO as a vehicle control or with various concentrations of EESR for 48 hours. - Cells are fixed with cold 70% ethanol for 3 hours at −20°C

Protocol tips
- After seeding cells are treated with 0.1% DMSO as a vehicle control or with various concentrations of EESR for 48 hours. - Cells are fixed with cold 70% ethanol for 3 hours at −20°C

Publication protocol

The effect of EESR on the cell cycle in HT29 cells was examined using the MuseTM Cell Cycle kit (Merck Millipore, Darmstadt, Germany) according to the manufacturer’s instructions. Briefly, cells (2 × 105 cells/well) were plated in 6-well plate and treated with 0.1% DMSO as a vehicle control or with various concentrations of EESR for 48 hours. The cells were then harvested, washed once with PBS, and fixed in cold 70% ethanol for 3 hours at −20°C. Fixed cells were centrifuged at 300 × g for 5 minutes and resuspended in PBS. After addition of an equal volume of MuseTM Cell Cycle reagent, cells were incubated for 30 minutes at room temperature in the dark. Finally, flow cytometry was conducted (MuseTM Cell Analyzer; Merck Millipore) and the Muse analysis software (ver 1.4) was used to determine the relative DNA content.

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Manufacturer protocol

Download the product protocol from Merck Millipore for Muse® Cell Cycle Assay Kit below.

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