Muse® Cell Cycle Assay Kit

Cell cycle assay human - HT-29

Experiment
Cell cycle assay human - HT-29
Product
Muse® Cell Cycle Assay Kit from Merck Millipore
Manufacturer
Merck Millipore

Protocol tips

Protocol tips
- After seeding cells are treated with 0.1% DMSO as a vehicle control or with various concentrations of EESR for 48 hours.

- Cells are fixed with cold 70% ethanol for 3 hours at −20°C

Publication protocol

The effect of EESR on the cell cycle in HT29 cells was examined using the MuseTM Cell Cycle kit (Merck Millipore, Darmstadt, Germany) according to the manufacturer’s instructions. Briefly, cells (2 × 105 cells/well) were plated in 6-well plate and treated with 0.1% DMSO as a vehicle control or with various concentrations of EESR for 48 hours. The cells were then harvested, washed once with PBS, and fixed in cold 70% ethanol for 3 hours at −20°C. Fixed cells were centrifuged at 300 × g for 5 minutes and resuspended in PBS. After addition of an equal volume of MuseTM Cell Cycle reagent, cells were incubated for 30 minutes at room temperature in the dark. Finally, flow cytometry was conducted (MuseTM Cell Analyzer; Merck Millipore) and the Muse analysis software (ver 1.4) was used to determine the relative DNA content.

Full paper   Login or join for free to view the full paper.

Reviews

Muse® Cell Cycle Assay Kit from Merck Millipore has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell cycle assay human - HT-29 using Muse® Cell Cycle Assay Kit from Merck Millipore.

Paper title
Extract Exhibits Antioxidant and Anticancer Activities by Inducing Cell Cycle Arrest and Apoptosis in Human Colon Adenocarcinoma HT29 Cells
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Merck Millipore for Muse® Cell Cycle Assay Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell cycle assay human - HT-29 using Muse® Cell Cycle Assay Kit from Merck Millipore. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms