Live/Dead Double Staining Kit (Merck)

Protocol tips

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	Incubate for 15 min at 37 ° C

Protocol tips
Incubate for 15 min at 37 ° C

Publication protocol

After calculating TC-50 values of each sample, live-dead assay was performed to confirm 50% cell mortality. The cells were exposed to particles with their respective TC-50 value. L-132 cells were exposed to nano and micron ZnO particles at concentration of 37.67 μg/mL and 35.04 μg/mL, respectively, for 24 h; similarly THP-1 cells were exposed to nano and micron ZnO particles at concentration of 17.69 μg/mL and 38.35 μg/mL, respectively, for 24 h. Likewise, L-132 cells were exposed to nano SiO2 at concentration of 312.5 μg/mL for 24 h while THP-1 cells were exposed to 17.69 μg/mL and 38.35 μg/mL of nano and micron SiO2 for 24 h, respectively. Control and particle interacted cells were stained using Live/Dead double staining kit (Calbiochem, Germany) as per the manufacturer’s instructions and then visualized under fluorescence microscope (Carl Zeiss, Germany).

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