Live-Dead Cell Staining Kit (BioVision)

Live / Dead assay mammalian cells - HepG2

Experiment
Live / Dead assay mammalian cells - HepG2
Product
Live-Dead Cell Staining Kit (BioVision) from Biovision
Manufacturer
Biovision

Protocol tips

Protocol tips
Incubate for 15 min at 37°C
Downstream tips
Observe cells immediately under a fluorescence microscope using a band-pass filter (detects fluorescein and rhodamine).

Publication protocol

The damage to tumor cells induced by thermal ablation with SWCNTs-(KFKA)7 and NIR irradiation was evaluated in vitro. Colon26 cells and HepG2 cells (2 × 105 cells/500 µl) were seeded in an 8-well chambered coverglass (Asahi Glass, Tokyo, Japan) and incubated overnight. After changing the culture medium, 1.5 µl and 5 µl of SWCNTs solution (200 µg SWCNTs/ml) was added to the 400 µl of culture medium of colon26 cells and HepG2 with final concentrations of 0.75 µg/ml and 2.5 µg/ml, respectively. After 2 hours of incubation, the wells were exposed to irradiation with an 808 nm NIR laser for 3 min at 1.2 W, collected in a 1.5 ml tube, and stained with a Live-Dead cell staining kit (Biovision, Mountain View, CA). The fluorescence microscopic observation was performed using a Biozero Bz-8000 (Keyence, Osaka, Japan) with Ex/Em = 470/535 nm (Live-Dye fluorescing green) and Ex/Em = 540/605 nm (propidium iodide fluorescing red), respectively. Confocal microscopy was carried out with A1RMP (Nikon, Tokyo, Japan) with Ex/Em = 488/525 nm (green) and Ex/Em = 562/595 nm (red), respectively. For flowcytometric analysis, cells were stained with propidium iodide of a Live-Dead cell staining kit and the number of labeled cells was analyzed by a FACSCant II (BD biosciences, San Jose, CA) with Ex/Em = 488/585 nm.

Full paper   Login or join for free to view the full paper.

Reviews

Live-Dead Cell Staining Kit (BioVision) from Biovision has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Live / Dead assay mammalian cells - HepG2 using Live-Dead Cell Staining Kit (BioVision) from Biovision.

Paper title
Photothermal ablation of tumor cells using a single-walled carbon nanotube-peptide composite.
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Biovision for Live-Dead Cell Staining Kit (BioVision) below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Live / Dead assay mammalian cells - HepG2 using Live-Dead Cell Staining Kit (BioVision) from Biovision. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms