Zombie Fixable Viability™ Sampler Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Dilute between 1:100-1:1000 dilution per 1-10 million cells in 100 µl volume	Incubate the cells at room temperature, in the dark, for 15-30 minutes

Upstream tips
Dilute between 1:100-1:1000 dilution per 1-10 million cells in 100 µl volume
Protocol tips
Incubate the cells at room temperature, in the dark, for 15-30 minutes

Publication protocol

Cells were blocked with normal rat serum (1:20 in PBS) and stained with the appropriate antibodies (Table S1) at 4°C for 30 min. Cells were stained using fixable live/dead cell stain kits (Invitrogen or BioLegend) and antibodies for surface antigens and then further processed using a Mouse Regulatory T Cell Staining kit (no. 2; eBioscience) according to the manufacturer’s protocol. All experiments were analyzed on a cell analyzer (FACS Canto II; BD), and the sample data were recorded using FACSDiva software (BD) and analyzed using FlowJo software (Tree Star).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Live / Dead assay mammalian cells - HEK 293 using Zombie Fixable Viability™ Sampler Kit from BioLegend.

Manufacturer protocol

Download the product protocol from BioLegend for Zombie Fixable Viability™ Sampler Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Live / Dead assay mammalian cells - HEK 293 using Zombie Fixable Viability™ Sampler Kit from BioLegend. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.