LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells

Protocol tips

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	Add 5 μL of 4 mM calcein-AM solution. Incubate the cells for 45 minutes at room temperature.

Protocol tips
Add 5 μL of 4 mM calcein-AM solution. Incubate the cells for 45 minutes at room temperature.

Publication protocol

A LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells (Thermo Fisher Scientific, Walthan, MA, USA) was used according to the manufacturer’s instructions to visualize cell viability. Briefly, 20 μL of 2 mM ethidium homodimer-1 (EthD-1) stock solution and 5 μL of 4 mM calcein-AM solutions were diluted in 10 mL of sterile and prewarmed Dulbecco’s Phosphate-Buffered Saline (DPBS, Welgene). The mixture was then mixed and distributed to cells in 96-well plates, followed by incubation for 45 min at room temperature. After the staining procedure, stained cells were imaged using fluorescent microscope (Nikon, ECLIPSE Ts2, Tokyo, Japan).

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