Live-Dead Cell Staining Kit (BioVision)

Protocol tips

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	Collect cells by centrifugation and resuspend to 0.5 ml Staining Solution. Incubate for 15 min at 37°C

Protocol tips
Collect cells by centrifugation and resuspend to 0.5 ml Staining Solution. Incubate for 15 min at 37°C

Publication protocol

The interaction of the cells with the CNCs was evaluated using the live-dead cell staining kit
(Biovision). The assay protocol has been described in previous studies.50, 51 Cell suspension (105
cells/mL) was deposited in 24-well plates and cultivated for 1, 3, 5 and 7 days. In parallel, different
concentration of CNCs (25, 50, 100 and 200 μg/mL Fe) was added in the cell suspension for every
different culture period. Cells that remained alive after certain days of cultivation were stained
green whilst dead cells were stained yellow-red. The images were recorded in a Zeiss fluorescence
microscope equipped with a Laser scanning system Radiance 2100 (400-700 nm) and a Carl-Zeiss
Axio Camera HR. Experiments were repeated at least three times for each size and available
concentration of the CNCs

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Manufacturer protocol

Download the product protocol from Biovision for Live-Dead Cell Staining Kit (BioVision) below.

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