CellTiter-Glo® Luminescent Cell Viability Assay

Protocol tips

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	Mix contents for 2 minutes on an orbital shaker to induce cell lysis. Allow the plate to incubate at room temperature for 10 minutes to stabilize luminescent signal.

Protocol tips
Mix contents for 2 minutes on an orbital shaker to induce cell lysis. Allow the plate to incubate at room temperature for 10 minutes to stabilize luminescent signal.

Publication protocol

MC3T3-GFP and MC3T3-Rorβ-GFP cell lines were seeded in growth medium into 96-well plates at a density of 2 × 104 cells/cm2 (n=6) and allowed to proliferate for 48 hours. Twenty-five (25) μl of conditioned media was assayed using the CellTiter-Glo® Luminescent Cell Viability Assay (Promega) according to the manufacturer’s instructions. The plate was read on a GloMax® luminometer (Promega) and data expressed as percent of MC3T3-GFP control.

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Manufacturer protocol

Download the product protocol from Promega for CellTiter-Glo® Luminescent Cell Viability Assay below.

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